Journal: FEBS Open Bio

Article Title: DDR2 signaling and mechanosensing orchestrate neuroblastoma cell fate through different transcriptome mechanisms

doi: 10.1002/2211-5463.13798

Figure Lengend Snippet: Schematic illustration of experimental process to find novel genes involved in biophysical stimulation. (A) The four sample conditions used in this study: shCTRL and shDDR2 cells cultured on 2 and 20 kPa collagen‐coated polyacrylamide gel substrates. (B) Experimental process of RNA‐seq: a 24‐h incubation of the cells on PAA gels followed by cellular RNA extraction, library construction, sequencing, and data analysis. This figure is created with BioRender.com .

Article Snippet: Polyacrylamide gel substrates were prepared through the polymerization of acrylamide (Bio‐Rad, Waltham, MA, USA, Cat. No. 1610140) and bis‐acrylamide, with varying concentrations to achieve the desired stiffness levels (Table ).

Techniques: Cell Culture, RNA Sequencing, Incubation, RNA Extraction, Sequencing